3-D Cell Culturing

3-Day Training Workshop

During this 3-D cell culturing workshop the researcher will learn how to successfully culture primary cells, cancer cells, and epithelial cells in a 3-D format using 3-D cell culture matrices, such as Collagen I. Emphasis will be placed on setting up primary human hepatocyte spheroid and high-throughput gut-epithelium-on-a-chip cultures, visualizing their formation and growth, and performing a broad range of downstream assays.  Examples of applications in toxicology and drug efficacy will be explored.

Overview of lecture topics:

3D Cell Culture Concepts

• Building 3-D Culture Models
• Cell Sourcing, Media
• Extracellular Matrices in 3D Cell Culture
• PPH Spheroids Tox
• Considerations in the Development and Use of MPS

3D Cell Culture Applications

• Operationalization use of Complex in vitro Models
• Disease modeling JHU on Neural & Neuro Disease Models
• Imaging in 3D – Practical Considerations