During this hands-on 3-D cell culturing workshop, the researcher will learn how to successfully culture primary cells, cancer cells, and epithelial cells in a 3-D format using 3-D cell culture matrices, such as Collagen I. Emphasis will be placed on setting up primary human hepatocyte spheroid and high-throughput gut-epithelium-on-a-chip cultures, visualizing their formation and growth, and performing a broad range of downstream assays. Examples of applications in toxicology and drug efficacy will be explored.
