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Antibody Validation and Characterization — 2 Day Training Workshop
December 12, 2022 @ 9:00 AM – December 13, 2022 @ 5:00 PM
This two day program is ideal for basic research and translational biology scientists who work with antibodies on a regular basis. This program which focuses on the types of artifacts associated with antibodies and introduce them to a variety of validation methods that can be implemented in research and academic laboratories.
Antibodies, and in particular monoclonal antibodies, are the single most important laboratory reagent, but are now also recognized as a main contributor to the reproducibility crisis that wastes research funding, undermines trust in scientific research, and jeopardizes scientific careers. This workshop brings to the participants’ attention the various factors that can compromise the validity of data produced with antibodies.
When it comes to verifying the integrity and identity of laboratory reagent, the burden of proof is increasingly being laid on researchers, and thus, the end-user of the reagents. The validation or authentication of antibodies can be performed with techniques and equipment readily available in many laboratories. Therefore, this workshop is recommended for anyone who relies on antibodies in their research.
- Reagent authentication: why now? Which reagents have the most “issues”?
- Brief intro to producing antibodies – what can go wrong?
- Antibody batch-to-batch variations and QC
- Problems introduced through antibody labeling
- Problems introduced by antibody purification
- Verifying antibody identity: suitable methods already available in most immunology labs
- Proper maintenance of hybridoma cells, proper storage of antibodies
Hands-on Lab Exercises:
- Antibody Validation by Immunoprecipitation/SDS-PAGE/Western Blotting
- Antibody Validation by Flow Cytometry
- Validating Antibody Avidity with Biosensors
” I thoroughly enjoyed this comprehensive and practical presentation of Flow Cytometry – Went back to my home lab and hit the ground running. Thanks for the top-quality training, Bio-Trac!”Gwenn Merry
FDA NCTR DSB BAMB
“This was a great course. The instructors were very knowledgable & helpful. Without introducing any bias towards particular commercial vendors, the course managed to provide a well balanced flavor for what is out there in terms of options for flow machines and software. The hands on workshops were also very helpful with truly individualized supervision during the classes.”Shaon Sengupta
University of Pennsylvania