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BIOL 055 – Genome Editing with CRISPR
August 2, 2021 @ 9:00 AM – August 6, 2021 @ 5:00 PM
This course will provide an overview of the current major genome engineering platforms and cover CRISPR genome editing technology in depth.
The first part of the course will focus on the general principles of genome editing technology including design, choice of format, delivery, efficiency, specificity, clonal isolation, genotyping, and validation. The second part of the course will address different applications including genome editing in mice, zebrafish, and induced pluripotent stem (iPS) cells, disease modeling, generation of reporter lines, and high throughput approaches. We will discuss strategies to make CRISPR gene editing more efficient, flexible, and specific. We will explore recent advances in the CRISPR field including base editors, prime editing, epigenome editing and microbial genome editing. We will also examine sequencing and quality control considerations for genome editing projects. Hands-on training on in-silico tools and carefully designed team projects will accompany the lecture material to provide practical training in design, assembly, transfection, and detection/evaluation steps of a typical genome editing workflow.