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Exosomes: Principles, Methods and Applications — 4 Day Training Workshop

November 7, 2022 @ 9:00 AM November 10, 2022 @ 5:00 PM


Taught by leaders in the field, this 4-day class provides students with an exciting, up-to-date introduction to the field of exosomes, microvesicles, and other extracellular vesicles (EVs). Lectures cover our current understanding of the biochemistry, molecular, and cell biology of EV biogenesis and uptake, the functions and analysis of EV RNAs, and the latest approaches to purifying and analyzing exosomes and other EVs. These lectures are interwoven with hands-on laboratory instruction in which participants use well-validated protocols, reagents and kits from major manufacturers to purify exosomes and interrogate their protein and RNA content. Participants will leave the class with the conceptual framework and practical know-how necessary to explore the contribution of exosomes and other EVs to whatever biological question they are investigating.

Lectures Topics:

Lectures will include understanding the expanding roles of exosomes in normal physiology and disease processes, as well as the development of new diagnostic tests and potential therapies using exosomes as well as exosome engineering and biomarker discovery.

  • Molecular Mechanisms Involved in the Biogenesis, Release and Uptake of Exosomes and Micro Vesicles and Their Role in Cell to Cell Signaling
  • Effect of Viral Infection on Exosome Production: Lessons from HIV and Other RNA Viruses Controlling Parthenogenesis;
  • Exosome Engineering
  • Exosomal microRNAs: An Introduction to Intracellular Social Media
  • Role of Exosomes in Regenerative Medicine and the Challenges that Remain
  • Exosomes from HTLV-1 and Ebola Infected Cells: Effects on Donor and Recipient Cells
  • Diagnostic use of Exosomes in Disease States/Novel Biomarker Discovery from Analysis of Exosomes from Patient Samples
  • Tracking and Monitoring Extracellular Vesicles in vivo;

Laboratory Topics:

  • Exosome Purification; Surface Exosome Markers (cd6-/-, 9-/-, 81-/-)
  • Exosome Isolation – Izon Sec Column, Centricon Spin
  • NTA: assay exosome preps for concentration, immunophenotype; Exoview camera – start overnight chip incubation
  • ATCC Repair Assay – Scratch Assays – Msc Exosomes, Cancer Exosomes
  • miRNA Profiling, Western blot, qPCR
  • 3D Culture Neutrosphere EVs, CN
  • Oxford Nano Systems – Surface Labeling Demonstration
  • Visualization – mRNA encoding a fluorescent protein/luciferase, then add to cells and assay for uptake and expression of the mRNA into fluorescent protein


November 7, 2022 @ 9:00 AM
November 10, 2022 @ 5:00 PM
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“Great investment. Took material that would have taken months to compile and perfect on my own, and packed it into 3 days.”

Richard Barrett
PHD Student
University of Central Florida

“Very informative workshop with a very knowledgable lecturer. Covered the various aspects of NGS and provided guidance I needed.”

Marquita Gittens-St. Hilaire
University of the West Indies (Cave Hill)
NGS 10/16


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20200 Observation Drive, Room 151/152
Germantown, MD 20876 United States
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