Gene Editing with CRISPR — 3 Day Training Workshop

This rigorous three day program is ideal for basic research and translational biology scientists who are looking for a balanced theoretical vs. hands-on introduction to CRISPR toolkit. Taught by active researchers, this workshop features several cutting-edge presentations delivered by experts in the CRISPR/Cas9 field along with an intensive schedule of laboratory exercises. This program will focus on CRISPR principles, strategies for successful project design, available expertise and resources, as well as a wealth of CRISPR technology diverse applications.

Lectures:

• Introduction into Basic Principles of Genome Editing Technologies
• Mechanisms of Double-Stranded DNA Break Repair
• Structural Aspects of Target Recognition by CRISPR/Cas9/sgRNA Complexes
• Biologic Diversity of Targeted Nucleases
• Comparative Assessment of CRISPR and RNAi Technologies
• Development of In Vitro/Cell Culture Models using CRISPR
• Engineering Mouse Models via CRISPR Mutagenesis for Basic and Translational Research and as an Experimental Platform for Preclinical Drug Development
• CRISPR/Cas9 Applications for Genome Editing in Pluripotent Stem Cells
• CRISPR/Cas9 Libraries and Their Unparalleled Value in Functional Screening and Gene Discovery Studies
• Emerging Clinical Applications of CRISPR/Cas9 Technology as a Promising Gene Therapy/Mutation Correction Strategy
• Bioinformatics Resources and Reagent Sources to Facilitate Successful Design and Implementation of CRISPR Projects.
• Ethical and Biological Safety Aspects of CRISPR/Cas9 Technology

Laboratory Exercises and Discussions:

• CRISPR/Cas9, ZFN, and TALEN Experimental Strategy Considerations;
• Gene Knockout and Validation with CRISPR/Cas;
• SURVEYOR/CEL-1 Assay: Genomic DNA Purification and CEL-I PCR Set-Up, Re-Annealing and Digestion Steps, PCR Gel Loading and Analysis, Troubleshooting, Interpretation
• Purification of Plasmid DNA and Genomic DNA from Cells and Tissue Biopsies
• Allele-Specific Genotyping Design and Practical Exercises
• Construction of sgRNA Containing Plasmid, In Vitro Synthesis, Purification, and Quality Control of sgRNA’s
• Introduction of CRISPR Reagents into Cultured Cells by Nucleofection
• Interactive Discussions on Best Practices in CRISPR/Cas9 Experimental Designs
• Exercises for Target Site Selection and sgRNA Design

“As always, Bio-Trac not only met my expectations, but exceeded them! I was immensely impressed with the class – both the lab work and lectures! I will absolutely be taking more classes with BioTrac – their classes should be required for all graduates entering the field of science. Easy to understand & follow, yet the classes get to the core of the discussed principle. I loved learning about CRISPR and would highly recommend this class to anyone in the field!”

Jennie Rowell PhD, RN
Assistant Professor, The Ohio State University
CRISPR

“Very informative workshop with a very knowledgable lecturer. Covered the various aspects of NGS and provided guidance I needed.”

Marquita Gittens-St. Hilaire
University of the West Indies (Cave Hill)
NGS 10/16