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Gene Editing with CRISPR — 3 Day Training Workshop

November 2, 2022 @ 9:00 AM November 4, 2022 @ 5:00 PM


This rigorous three day program is ideal for basic research and translational biology scientists who are looking for a balanced theoretical vs. hands-on introduction to CRISPR toolkit. Taught by active researchers, this workshop features several cutting-edge presentations delivered by experts in the CRISPR/Cas9 field along with an intensive schedule of laboratory exercises. This program will focus on CRISPR principles, strategies for successful project design, available expertise and resources, as well as a wealth of CRISPR technology diverse applications.


  • Introduction into Basic Principles of Genome Editing Technologies
  • Mechanisms of Double-Stranded DNA Break Repair
  • Structural Aspects of Target Recognition by CRISPR/Cas9/sgRNA Complexes
  • Biologic Diversity of Targeted Nucleases
  • Comparative Assessment of CRISPR and RNAi Technologies
  • Development of In Vitro/Cell Culture Models using CRISPR
  • Engineering Mouse Models via CRISPR Mutagenesis for Basic and Translational Research and as an Experimental Platform for Preclinical Drug Development
  • CRISPR/Cas9 Applications for Genome Editing in Pluripotent Stem Cells
  • CRISPR/Cas9 Libraries and Their Unparalleled Value in Functional Screening and Gene Discovery Studies
  • Emerging Clinical Applications of CRISPR/Cas9 Technology as a Promising Gene Therapy/Mutation Correction Strategy
  • Bioinformatics Resources and Reagent Sources to Facilitate Successful Design and Implementation of CRISPR Projects.
  • Ethical and Biological Safety Aspects of CRISPR/Cas9 Technology

Laboratory Exercises and Discussions:

  • CRISPR/Cas9, ZFN, and TALEN Experimental Strategy Considerations;
  • Gene Knockout and Validation with CRISPR/Cas;
  • SURVEYOR/CEL-1 Assay: Genomic DNA Purification and CEL-I PCR Set-Up, Re-Annealing and Digestion Steps, PCR Gel Loading and Analysis, Troubleshooting, Interpretation
  • Purification of Plasmid DNA and Genomic DNA from Cells and Tissue Biopsies
  • Allele-Specific Genotyping Design and Practical Exercises
  • Construction of sgRNA Containing Plasmid, In Vitro Synthesis, Purification, and Quality Control of sgRNA’s
  • Introduction of CRISPR Reagents into Cultured Cells by Nucleofection
  • Interactive Discussions on Best Practices in CRISPR/Cas9 Experimental Designs
  • Exercises for Target Site Selection and sgRNA Design


November 2, 2022 @ 9:00 AM
November 4, 2022 @ 5:00 PM
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“As always, Bio-Trac not only met my expectations, but exceeded them! I was immensely impressed with the class – both the lab work and lectures! I will absolutely be taking more classes with BioTrac – their classes should be required for all graduates entering the field of science. Easy to understand & follow, yet the classes get to the core of the discussed principle. I loved learning about CRISPR and would highly recommend this class to anyone in the field!”

Jennie Rowell PhD, RN
Assistant Professor, The Ohio State University

“Very informative workshop with a very knowledgable lecturer. Covered the various aspects of NGS and provided guidance I needed.”

Marquita Gittens-St. Hilaire
University of the West Indies (Cave Hill)
NGS 10/16


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20200 Observation Drive, Room 151/152
Germantown, MD 20876 United States
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