Generating The Neural Lineage From iPSCs
October 25 @ 9:00 AM – October 28 @ 5:00 PM
This four-day workshop is ideal for researchers interested in developing neural lineages from iPSCs. Team taught by active researchers from Johns Hopkins, the NIH, the FDA, and Thermo Fisher Scientific, participants will have the opportunity to gain a solid foundation in iPSC methods with emphasis on culturing induced pluripotent stem cells (iPSCs) and differentiating to different neural lineage in both adherent and 3D systems. Functional assays will be discussed in lectures for assessing neuronal differentiation. Applications of iPSC-derived neural lineages will be discussed to and their use in neuropharmacology, toxicity testing, and therapy.
- Overview of Stem Cell Biology
- Advances in Stem Cell Feeder Free Culture: StemFlex and StemScale Media
Lab: PSC Culture
- Examine PSC 2D/adherent Cultures and Clump Passage.
- Cryopreserve PSCs (Demo).
- Dissociate Monolayer Culture and Establish 3D PSC Suspension Culture with +/- Rock Inhibitor
“Great investment. Took material that would have taken months to compile and perfect on my own, and packed it into 3 days.”Richard Barrett
University of Central Florida
“It was my first experience with professional development workshop in the USA. I liked it a lot. It was well organized, flexible and friendly. It helped to put in order my preceding knowledge and gain more.”Larisa Ryzhara, MD, PhD
Staff Scientist II, Maine Medical Center Research Institute