Histochemical Society Immunohistochemistry & Immunofluorescence Workshop
June 11 – June 15
This 4.5-day laboratory short course is ideal for experimental and translational research scientists, diagnosticians, and technical staff who are new to immunohistochemistry and immunofluorescence or those with experience who aim to take their skills to the next level. Our faculty are expert practitioners with diverse backgrounds in terms of the theory and application of these techniques as well as microscopy. Faculty to student ratios are low (1:3 or better) to maximize participants’ experience at the course and foster networking opportunities during and beyond the course. Lunch and dinner will be included. Note: Travel awards (up to $500) are available through the Histochemical Society for this event.
Topics include: Pre-analytics: Sample acquisition, fixation, processing embedding and microtomy; Reagents and validation: Reagents, antibody validation, protocol optimization and controls; Fundamentals of labels and stains: color, chromogen and fluorescent detection systems; Microscopy basics: transmitted light and fluorescence microscopes, limitations of the microscope and slide; Troubleshooting sessions; Advanced immunohistochemistry topics: antigen retrieval, multiplex immunohistochemistry, other detection systems and molecular targets; Horizon Lectures – automated IHC, quantitative digital pathology, digital imaging and deep learning, highly multiplexed IHC and TBA
Hands-on Laboratory: Immunohistochemistry laboratory; Dual label immunofluorescence laboratory; Microscopy and Digital Imaging;