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Immunohistochemistry & Immunofluorescence: A Histochemical Society Hands-on Training Course

June 8, 2022 @ 8:00 AM June 12, 2022 @ 12:00 PM


This 4.5-day laboratory short course is ideal for experimental and translational research scientists, diagnosticians, and technical staff who are new to immunohistochemistry and immunofluorescence or those with experience who aim to take their skills to the next level. Our faculty are expert practitioners with diverse backgrounds in terms of the theory and application of these techniques as well as microscopy. Faculty to student ratios are low (1:3 or better) to maximize participants’ experience at the course and foster networking opportunities during and beyond the course. Lunch and dinner will be included.


  • Pre-analytics: Sample acquisition, fixation, processing embedding and microtomy
  • Reagents and validation: Reagents, antibody validation, protocol optimization and controls
  • Fundamentals of labels and stains: color, chromogen and fluorescent detection systems
  • Microscopy basics: transmitted light and fluorescence microscopes, limitations of the microscope and slide
  • Faculty facilitated discussions
  • Troubleshooting sessions
  • Advanced immunohistochemistry topics: antigen retrieval, multiplex immunohistochemistry, other detection systems and molecular targets
  • Horizon Lectures – automated IHC, quantitative digital pathology, digital imaging and deep learning, highly multiplexed IHC and TBA

“The course was a really great boot camp for getting the fundamentals of IHC and microscopy. I have adopted many of the protocols and practices in my regular lab work and I appreciate having the background on WHY the protocol has the steps that it does in staining. It really helps to troubleshoot when you understand the mechanism. I highly recommend this course to anyone looking to build a solid foundation in IHC and microscopy.”

Ginny Schultz
Research Lab Supervisor at Seattle Children’s Research Institute


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20200 Observation Drive, Room 151/152
Germantown, MD 20876 United States
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