4-Day Training Workshop
November 19 @ 9:00 AM – November 22 @ 5:00 PM
This four day program is ideal for those who are looking for an in-depth, hands-on introduction to Flow Cytometry. The instructors will focus on a broad spectrum of flow cytometric topics, discuss examples from various research applications, provide technical protocols for flow cytometric sample preparation, as well as data analysis, trouble shooting and experimental design. Attendees will have hands-on experience on three different flow platforms (Becton Dickinson, Miltenyi, Bio-Rad and Sony) as well as exposure to four software analysis programs.
Overview of the lecture:
Lectures:
Basic Analytical Techniques, Principles, and Trouble Shooting; Principles of Cell Staining, Choosing the Right Experimental Controls, Instrument Setup (baseline and compensation); Phenotyping and Analyzing Cell Populations by Flow Cytometry – How to present and interpret flow cytometric data; Introduction to acquisition and analysis Software; Purification and Labeling of Antibodies for Flow Cytometric and Other Applications; Flow Sorting: Critical Experimental Issues and Instrument Setup; Parameters; Functional Flow Cytometric Assays;
Laboratory:
Preparation of Samples for Multicolor Analysis Flow Sorting; Multicolor Analysis, Compensation and Flow Sorting; Hands-on Flow Cytometry Exercises Using Miltenyi MACSQuant Analyzer; Bio-Rad S3e flow sorter; BD Accuri C6; Software training: Introduction to FlowJo Cytometry Analysis Software; Introduction to Cyflogic Cytometry Analysis Software; Flow Cytometry – Acquisition/Trouble Shooting/ Software; Introduction to FCS Express Cytometry Analysis Software
Attendees attend all lectures and labs listed.