Bio-Trac: Gene Editing with CRISPR

This rigorous three-day program is ideal for basic research and translational biology scientists who are looking for a balanced theoretical vs. hands-on introduction to CRISPR toolkit. Taught by active researchers, this workshop features several cutting-edge presentations delivered by experts in the CRISPR/Cas9 field along with an intensive schedule of laboratory exercises. This program will focus on CRISPR principles, strategies for successful project design, available expertise and resources, as well as a wealth of CRISPR technology diverse applications.

Overview

Lectures: 

• Introduction into Basic Principles of Genome Editing Technologies
• Mechanisms of Double-Stranded DNA Break Repair
• Structural Aspects of Target Recognition by CRISPR/Cas9/sgRNA Complexes
• Biologic Diversity of Targeted Nucleases
• Comparative Assessment of CRISPR and RNAi Technologies
• Development of In Vitro/Cell Culture Models using CRISPR
• Engineering Mouse Models via CRISPR Mutagenesis for Basic and Translational Research and as an Experimental Platform for Preclinical Drug Development
• CRISPR/Cas9 Applications for Genome Editing in Pluripotent Stem Cells
• CRISPR/Cas9 Libraries and Their Unparalleled Value in Functional Screening and Gene Discovery Studies
• Emerging Clinical Applications of CRISPR/Cas9 Technology as a Promising Gene Therapy/Mutation Correction Strategy
• Bioinformatics Resources and Reagent Sources to Facilitate Successful Design and Implementation of CRISPR Projects.
• Ethical and Biological Safety Aspects of CRISPR/Cas9 Technology

Laboratory Exercises and Discussions:

• CRISPR/Cas9, ZFN, and TALEN Experimental Strategy Considerations;
• Gene Knockout and Validation with CRISPR/Cas;
• SURVEYOR/CEL-1 Assay: Genomic DNA Purification and CEL-I PCR Set-Up, Re-Annealing and Digestion Steps, PCR Gel Loading and Analysis, Troubleshooting, Interpretation
• Purification of Plasmid DNA and Genomic DNA from Cells and Tissue Biopsies
• Allele-Specific Genotyping Design and Practical Exercises
• Construction of sgRNA Containing Plasmid, In Vitro Synthesis, Purification, and Quality Control of sgRNA’s
• Introduction of CRISPR Reagents into Cultured Cells by Nucleofection
• Interactive Discussions on Best Practices in CRISPR/Cas9 Experimental Designs
• Exercises for Target Site Selection and sgRNA Design