Bio-Trac and ATCC Partner to Hold Mammalian Cell Culture Workshop

Workshop will provide comprehensive overview, hands on training in the principles and techniques of mammalian cell culture

Course to be team taught by experts with years of tissue culture experience

GERMANTOWN, Maryland, Jan. 10, 2023 – Bio-Trac and ATCC, the world’s premier biological materials management and standards organization, have partnered to conduct a “Best Practices in Mammalian Cell Culture” training workshop to offer a comprehensive overview of the principles and techniques related to cell culture techniques.

The course will be taught by cell culture experts from ATCC, NIST and Bio-Trac. Beyond the overview of mammalian cell culture, the workshop will also explore primary cell culture, organoids and 3D cultures, microphysiological systems, assay development, and cell line authentication.

“We are delighted to be working with Dr. Heather Branscome and ATCC’s team of experts on this initiative”, said Mark Nardone, Director and Co-Founder of Bio-Trac. “Proper cell culture technique is a foundation of scientific research; containing many principles and nuances that need to be followed to limit contamination and ensure reproducibility. There is hardly a Biological discipline that does not depend on the use of cell culture for their research.”

Topics discussed during the workshop include:

  • Introduction to Cell Culture/Aseptic Technique
  • Constituents of Tissue Culture (Media, Serum, etc.)
  • Principles and Considerations for Setting up a Tissue Culture Lab
  • Cell Line Authentication
  • Authentication of Non-Human Cell Lines. Isozymes, COI Multiplex Assay; SNPs, and STRs
  • Best practices in Cryopreservation of Mammalian Cells
  • Primary Cell Isolation and Culture
  • Organoids and 3D Cell Culture
  • Microphysiological systems
  • Cell Culture-based Assay Development for Gene Therapy Product Testing

Participants will receive practical, hands-on laboratory training exercises related to cell culture, including thawing, expansion cryopreservation and cell-based assays. The laboratory sessions will be guided by ATCC subject matter experts.

Laboratory topics include:

  • Aseptic Technique Demonstration
  • Media/Reagent Preparation
  • Thawing/counting/seeding Adherent Cell Line
  • Thawing/counting/seeding Suspension Cell Line
  • Observation and Reseeding of Suspension Cell Line
  • Observation and Passaging of Adherent Cell Line
  • Doubling Time and PDL Calculations
  • Set up Luciferase Reporter Cell-based Assay
  • Harvesting of Pre-plated Cells
  • Preparation of Cryopreservation Medium
  • Cryopreservation of Cells
  • Finish Luciferase Reporter Assay

“This course will combine both theory and practice to provide participants with a holistic experience of mammalian cell culture,” said Dr. Heather Branscome, Supervisor of Laboratory Operations at ATCC. “Cell culture is a fundamental and versatile tool that is important for anyone working in a cellular, molecular, or microbiology lab so it is imperative to have a strong foundation in the underlying principles.”

The workshop will be held Feb. 8-10 from 9 am to 5 pm at the MC Bioscience Education Center (BEC) in Germantown, MD. The program is limited to 18 participants. A registration fee of $995 is required. Mask requirements are currently in place in the BEC as well as social distancing measures. Proof of Covid-19 vaccination is required.

Special $104 per night hotel rates are available for Bio-Trac attendees at the Holiday Inn Express.

About Bio-Trac

Bio-Trac® Training Programs are hands-on training workshops, designed for research scientists, which focus on the latest relevant techniques that are necessary for laboratory research. Open to the scientific community, all workshops are team taught by experts, active researchers from prestigious government, academic and private research institutions. Established in 1985 at the National Institutes of Health, Bio-Trac has provided hands-on training workshops to the scientific community for over 37 years; training over 18,000 research scientists.

About ATCC

ATCC is a premier global biological materials and information resource and standards organization and the leading developer and supplier of authenticated cell lines, microorganisms, and associated data for academia, industry, and government. With a history of scientific contributions spanning nearly a century, ATCC offers an unmatched combination of being the world’s largest and most diverse collection of biological reference materials and data, and is a mission-driven, trusted partner that supports and encourages scientific collaboration. ATCC products, services, partnerships, and people provide the global scientific community with credible, advanced, model systems to support complex research and innovations in basic science, drug discovery, translational medicine, and public health. ATCC is a 501(c)3 nonprofit organization with headquarters in Manassas, Virginia, and a research and technology center of excellence in Gaithersburg, Maryland. 

About the Montgomery College Bioscience Education Center

The Bioscience Education Center (BEC), located on the Montgomery College Germantown, MD Campus, is a 125,000 sq/ft training facility containing 25 wet labs, smart classrooms as well as state-of-the-art lab equipment currently found in area biotech and biopharma companies.  Home of the MC Biotech program, the BEC provides scientific training at all levels, from early college students to MD/PhD research scientists.